Rehidratación y disgregación mecánica del reservorio de nematodos entomopatógenos steinernema glaseri para su liberación

Víctor Alberto Franco Luján; Adrián Martínez Vargas; Heriberto Cruz Martínez; Samuel Ramírez Arellanes; Jaime Ruiz Vega

doi:10.61117/ipsumtec.v6i6.52

Authors

Víctor Alberto Franco Luján Tecnológico Nacional de México https://orcid.org/0000-0002-9929-1770
Adrián Martínez Vargas Tecnológico Nacional de México https://orcid.org/0000-0002-0896-5195
Heriberto Cruz Martínez Tecnológico Nacional de México https://orcid.org/0000-0001-8967-1481
Samuel Ramírez Arellanes Tecnológico Nacional de México https://orcid.org/0009-0008-3928-4553
Jaime Ruiz Vega Tecnológico Nacional de México https://orcid.org/0000-0002-4259-4930

DOI:

https://doi.org/10.61117/ipsumtec.v6i6.52

Keywords:

Release, Nematodes, Infectivity, Pathogenicity

Abstract

Reservoirs with entomopathogenic nematodes (EPNs) have shown to be a viable alternative for their storage and survival; however, one disadvantage is their release from such reservoirs. Therefore, this work aims to evaluate the level of rehydration and mechanical disintegration of the reservoir for the release of EPNs Steinernema glaseri. The level of rehydration was achieved using six permanency times of reservoirs (0, 10, 20, 30, 40, and 50 s) in a nebulizer chamber. Subsequently, the maximum water absorption capacity and the microstructural cohesion of the reservoir were determined based on the level of rehydration. The mechanical disintegration was evaluated with four potential energies of 0.2, 0.4, 0.6, and 0.8 m.Finally, the infectivity of the EPNs released from the reservoir were evaluated on Galleria mellonella last instar larvae. The results showed that the reservoir needs a level of rehydration higher than 326.2 μL of water to begin its disintegration, having a water absorption capacity of 90.90%.The microstructural cohesion of the unrehydrated reservoir was 711 Pa, which increased with the level of rehydration, reaching a maximum value of 796 Pa. Likewise, the energies of 0.6 and 0.8 m were able to achieve a mechanical disintegration of the reservoir. A level of rehydration of 326.2 μL with a mechanical disintegration energy of 0.6 m caused the EPNs release of 100%, which achieved a maximum infectivity of 95% about Galleria mellonella. The results of this research show the feasibility of using reservoirs for the application of NEPs as biological control agents.

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